AssayStable Banner slide 03 AssayStable Banner slide 02 AssayStable Banner slide 01

PCRstable® & AssayStable®

All-Ambient Assays on Any Device

PCRstable and AssayStable are ambient temperature stability technologies that allows assay users and manufacturers to stabilize their assays in any flexible format using Biomatrica’s unique reagents with a simple air-dry process. PCRstable was developed for PCR-based assays, and AssayStable was developed for all other assays.

PCRstable and AssayStable technologies provide the following benefits:

Assay Manufacturers:

  • Air-dry any assay reagent in-house and store at room temperature
  • No lyophilization and no low humidity chambers needed
  • Virtually any reagent stabilized by patented stabilizers

Assay Users:

  • Prepare any assay in a dry-stable format
  • Add template and go; no mixing needed
  • Rapid rehydration upon template addition
AssayStable Overview Image

Stabilization of qPCR assay

Included in dry-down:

  • Taq
  • Primers
  • Reverse Transcriptase
  • Dye (SYBR® Green)/Taqman®
  • dNTPs
  • Salts and Detergent

  • Stabilization of qPCR assay at ambient temperature

    AssayStable qPCR image
    Figure 1: Components of qPCR assay, including Taq DNA polymerase, dNTPs, primers for RNase P, SYBR Green dye and PCR buffer were dried down in the presence of Biomatrica’s stabilizing formulation (Protected) or Non-Protected, and stored under high stress conditions at 45°C. At determined time points, samples were rehydrated with water and MgCl2 with the specified amount of genomic DNA template. Real time PCR amplification of a RNase P fragment was performed on the ABI 7300 Real-Time PCR System, using the equivalent amount of DNA with qPCR reagents stored at -20°C as a positive control. Data shown is for samples stored for 6 months at 45°C, corresponding to 2 years of ambient temperature storage, based on accelerated aging.


    Stabilization of RT-qPCR assay at ambient temperature

    AssayStable RT-qPCR image
    Figure 2: All components of a RT-qPCR assay, including Taq DNA polymerase with reverse transcriptase activity, dNTPs, aptamer and PCR buffer including metal co-factor were dried down in the presence of Biomatrica’s stabilizing formulation (Protected) or Non-Protected, and stored under high stress conditions at 45°C. At determined time points, samples were rehydrated with water, primers and TaqMan probes, and a known input of RNA template was added. Reverse transcription and real time PCR amplification were performed on ABI 7300 Real-Time PCR System, using the equivalent amount of RNA samples with RT-qPCR reagents stored at -20°C as a positive control. Data shown is for samples stored for 3 weeks at 45°C, corresponding to 3 months of ambient temperature storage, based on accelerated aging.

    qPCR Stabilized

    qPCR Stabilized Image
    Figure 3: Taq + dNTPs stabilized for 4 years at room temperature(based on accelerated aging).

    Stabilization of a Limiting Quantity of Reverse Transcriptase

    Stabilization of a Limiting Quantity of Reverse Transcriptase Image
    Figure 4: Stabilization of a limiting amount of reverse transcriptase for rapid screening ability.

    Ambient Shipping: Stress Testing

    Ambient Shipping Stress Testing Image
    Figure 5: Biomatrica stabilizers protected all samples, while non-protected shows complete degradation below 10 ng.

    Contact Us

    Biomatrica, Inc.
    5627 Oberlin Drive, Suite 120
    San Diego, CA 92121

    Tel: 858-550-0308
    Fax: 858-678-0597

    Or call us toll-free in North America:
    866-379-6879

    E-mail: info@biomatrica.com