DNAgard® Saliva HT

Saliva DNA Collection Optimized for Streamlined Automated Processing
  • Pierce-able cap eliminates time-consuming uncapping for automated processing
  • Triple-redundant labeling & LIMS-compatible for accurate sample tracking
  • User-friendly design increases protocol compliance

DNAgard Saliva HT

Product Description

DNAgard® Saliva HT is a saliva DNA collection device designed to collect and stabilize saliva samples for genetic analysis. Innovative design makes stabilized saliva collection simpler, less error prone, and seamlessly supports high-throughput automated platforms.

For laboratories, DNAgard® Saliva HT features a pierce-able cap that eliminates time-consuming de-capping steps along with triple redundant tracking labels that are LIMS-compatible label for accurate sample tracking.

For subjects DNAgard® Saliva HT provides a simplified collection process with a spill-proof design, tethered cap, and no additional reagents to mix in. These improvements eliminate user contact with the chemical stabilizer and accommodate arthritic hands.

DNAgard Saliva HT Workflow
For Research Use Only. Not for use in diagnostic procedures.

DNAgard® Saliva HT Preserves Saliva DNA Integrity and Delivers High PCR Yield After Long-Term Storage

Saliva samples from five (5) donors collected individually into conical tubes were aliquoted into tubes containing DNAgard® Saliva HT solution (DGS HT), Competitor O’s solution (CO), or in a conical tube with non–protective control solution (NP). All samples were stored at 45°C for five (5) weeks and moved to 60°C for an additional nine (9) days, equivalent to at least 10 months at 22°C calculated using the Arrhenius equation.

Preserved DNA integrity after 10 months at room temperature

DNAgard Saliva <em>HT</em> gel 10 month image
Figure 1: Agarose gel of DNA purified from saliva stored using Competitor O, DNAgard® Saliva HT, or non-protected saliva samples. DNA was visualized on a 1% agarose gel stained with ethidium bromide.

High DNA yield after 10 months at room temperature

Bar chart showing saliva DNA yield from 5 donors after storage in DNAgard Saliva HT, Competitor O stabilizer and non-protected
Figure-2: dsDNA yield of DNA isolated from saliva stored using Competitor O, DNAgard® Saliva HT, or non-protected saliva samples. DNA concentration was measured using Quant-iT™ PicoGreen® dsDNA Assay.

Summary of purity and yield results from DNA preserved in DNAgard® Saliva HT

DNgard Saliva HT Summary of  purity and yield results after 10 months of storage at room temperature based on accelerated stability conditions
Table-1: Summary of purity and yield results from DNA preserved in DNAgard® Saliva HT as indicated above.

Gel analysis of long-range PCR from saliva DNA preserved in DNAgard Saliva HT

Gel analysis image of long-range PCR from saliva DNA preserved in DNAgard Saliva HT
Fiture 3A 3.9 kb fragment of the human GAPDH gene was amplified from saliva DNA after storage in DNAgard® Saliva HT (DGS-HT), Competitor O (CO), and non-protected solution (NP). Primers specific to the human GAPDH gene were synthesized by Integrated DNA Technologies Inc. (Table 1). Long-range PCR was performed. Approximately 5% of the purified DNA from each sample was used in each reaction. 40% of the PCR products from each reaction were visualized on a 1% agarose gel stained with ethidium bromide

qPCR of saliva DNA preserved in DNAgard® Saliva HT

qPCR of human DNA stored using Competitor O, DNAgard® Saliva HT, or nonprotected saliva samples. Samples were amplified in duplicate using iQ™ SYBR® Green Supermix (Bio-Rad), on a CFX96 Real-Time PCR Instrument (Bio-Rad) using primers specific to the human RNase P gene.

Product Specifications

Saliva sample: 2ml
DNAgard® Saliva HT (Stabilizer): 2ml
DNA preservation at room temperature: 1 year
For Research Use Only. Not for use in diagnostic procedures.

Ordering

Catalog No. Product Description Size Price
97021-012A DNAgard® Saliva HT 48 kits $960.00

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