Preserve DNA in Saliva Instantly
DNAgard® Saliva allows for the efficient collection, preservation and shipping of saliva samples for DNA isolation and analysis. The product is designed for ease of use, with a simple process from collection to storage: (1) Spit into collection device, (2) Add the stabilizer to the collection device, (3) Remove the collection funnel from the device, (4) Screw on the cap, (5) Mix gently by inverting the tube 5 - 7 times. DNA in saliva samples are preserved for up to 2 years at room temperature, with high quality DNA recovery. The stabilizing preservative has been formulated to offer flexible DNA recovery from a wide range of DNA extraction and purification kits.
For Research Use Only. Not for use in diagnostic procedures.
Figure 1: Comparison of DNA stored in DNAgard Saliva (DGS) versus non-protected DNA (NP) and DNA stored at -80°C. Two hundred µl of saliva from one donor was mixed with 150 µl of DNAgard Saliva. The mixture was stored at room temperature for 2 years. Also, 200 µl aliquots of saliva from the same donor were stored non-protected (NP) at room temperature and at -80°C for the same time period as negative and positive controls. DNA was isolated using QIAamp® DNA Blood Mini Kit and eluted with 100 µl of elution buffer. Samples were analyzed by electrophoresis on a 0.8% agarose gel.
Figure-2: DNA purity analyzed by UV spectroscopy (A260/A280) DNA from the saliva of 3 donors preserved in DNAgard Saliva (DGS) has equivalent or greater purity than DNA from saliva of the same donors stored at -80°C.
Figure-3: DNA yield analyzed by PicoGreen® Assay Saliva DNA preserved in DNAgard Saliva(DGS) for 2 years at room temperature shows equivalent or greater yield than that of saliva DNA stored at -80°C for the same time period as measured by PicoGreen assay.
|Product Overview Sheet|
|Saliva Collection Protocol|
|Saliva DNA Purification Protocol|
|DNAgard® Saliva Poster|
|DNA purification from saliva stabilized in DNAgard® Saliva using the QIAamp® Blood Mini Kit|
|DNA purification from saliva stabilized in DNAgard® Saliva using an organic extraction method|
|DNA purification from saliva stabilized in DNAgard® Saliva using the Gentra® Puregene® Kit|
|Catalog No.||Product Description||Size|
|97021-011S||DNAgard® Saliva Sample Pack||5 kits|
|97021-011A||DNAgard® Saliva (10 - 100 kits)||10 - 100 kits|
|97021-011B||DNAgard® Saliva (101 - 1,000 kits)||101 - 1,000 kits|
|97021-011C||DNAgard® Saliva (>1,000 kits)||>1,000 kits|