AssayStable
  • Biomatrica DNA Products
  • Biomatrica RNA Products
  • Biomatrica Bacteria Products
  • Biomatrica Assay Enhancers
  • Biomatrica Software Products
Biomatrica Online Store
DNA Stable Plus A DNA Preservation Product

DNAgard® Tissue

Collect, Ship and Store Tissues & Cells at Room Temperature.

  • Protect DNA in tissues, cells and biopsy material from degradation at room temperature
  • Compatible with most downstream applications, e.g., PCR, qPCR and sequencing.
  • Optional dry-down feature allows for long-term storage
  • Reduces storage and shipping costs and improves lab space management

DNAgard® is designed for immediate stabilization of DNA from intact cells and tissues with the convenience of room temperature shipping, processing and storage. The liquid storage reagent rapidly permeates cell membranes to stabilize and protect genomic DNA.

DNAgard Workflow Overview

DNAgard Tissue Workflow Overview

Product Performance

DNA protection in rat kidney tissues at room temperature

Agarose gel of DNA from rat kidney tissues stabilized in DNAgard Tissue
Figure 1: Gel image showing genomic DNA isolated from rat kidney (25 mg) stored for 195 days in DNAgard, water (non-protected: NP) or frozen at -80°C was analyzed on 0.8% agarose gel. (M = 1 kb ladder).

DNAgard Tissue protects DNA in a variety of tissues

Gel image showing DNAgard protects DNA in rat heart, kidney, lung, spleen, tail, brain, and liver tissue.
Figure 2: Gel images showing genomic DNA isolated from a variety of rat tissues (25 mg) stored for 70 days in DNAgard Tissue, water (non-protected: NP) or frozen at -80°C was analyzed on a 0.8% agarose gel. (M = 1 kb ladder).

DNAgard protects DNA in mammalian cells at room temperature

Agarose gel showing DNAgard protects DNA in mammalian cells at room temperature
Figure 3: (A) 106 293T cells were pelleted and the supernatant (media) was removed. Cell pellets were resuspended in 500 µl of DNAgard or water (non-protected). For comparison, 106 cells were spotted on Whatman FTA paper and dried. As a positive control, 106 cells were stored at - 80°C in 10% DMSO. DNAgard, Whatman and non-protected samples were stored at room temperature. After 84 days of incubation samples were processed for DNA isolation using a commercially available column purification technology. (The DNA was eluted from the Whatman FTA samples using Whatman’s published protocol). The DNA yield and integrity from 5 x 104 293T cells was compared directly on a 0.8% agarose gel. (T’0’ is DNA recovered at the time cells were harvested; M = 1 kb ladder).
(B) Representative DNA yields from DNAgard samples compared to frozen controls. 1 DNA was isolated from tissue fragments or 106 mammalian 293T cells stored in liquid DNAgard for 2 months. DNA yield was quantified using the Quant-iT PicoGreen fluorescent assay (Invitrogen). Yield is presented as ng of DNA per mg tissue for the animal tissue samples or total DNA for the 293T cell samples.

 

Broad range of applications


Successful real-time PCR and long-range PCR after 2 months storage in DNAgard and at -80°C .

qPCR with genomic DNA recovered after 26 months in DNAstable
Figure 4: Rat kidney fragments were weighed (approx. 25 mg each), immediately submerged in DNAgard solution or water (NP), and stored at room temperature. Control samples were frozen and stored at -80°C. After 71 days, total DNA was recovered from the stored samples using a commercially available column purification technology. DNA was analyzed by real time PCR amplification of the beta-actin gene.

 

Unique dry-down feature for longer storage at room temperature

Figure 5: Dry stabilization of tissue culture cells in for 2 months at 50 °C (accelerated aging theory = 1 year at room temperature).
105 293T cells were pelleted and the supernatant (media) was removed. Cell pellets were resuspended in 500 µl of DNAgard or water (non-protected). The cells were then spotted on 96-well microtiter plates for drying in a sterile laminar flow hood. For comparison, 105 cells were spotted on Whatman FTA paper and dried. As a positive control, 105 cells were stored at -80°C in 10% DMSO. Dried samples were stored at room temperature or 50°C in heat-sealed pouches containing desiccant. After 65 days of incubation, the dried DNAgard and non-protected samples were rehydrated in 500 µl 1X PBS buffer. These samples, and the -80°C positive controls, were then processed for DNA isolation using a commercially available column purification technology. (The DNA was eluted from the FTA samples using a published protocol.) The DNA yield and integrity from 5 x 104 293T cells was compared directly on a 0.8% agarose gel.

Product Specifications


Product formats 3 x 500 µL tubes, 50 mL bottle, 100 mL bottle, Custom volumes
Application volume At least 100 µL per 10 mg tissue; 100 µL per 106 cells
DNA storage 6 months
DNA stabilization testing temperature 20°C
Resources
DNAgard® Tissue Overview
DNAgard® Tissue Handbook
DNAgard® Protocol
DNAgard® MSDS

 

 

Posters and Application Notes
Impact of collection, stabilization and isolation of bovine ear punches on high and low density genotyping arrays
Innovative technology to stabilize DNA at room temperature in tissues and cells
Application Note: Shipping Simulation

Ordering

Catalog No. Product Description Size
62001-036 DNAgard® Tissue 50 mL
62001-046 DNAgard® Tissue 100 mL
63020-016 DNAgard® Tissue Trial Kit 3 tubes x 500 µL
90028-290 96-well plates (empty) 5 plates

Content 6