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DNA Stable Plus A DNA Preservation Product

DNAstable®

Stabilize store and ship purified DNA at room temperature

  • Long-term stabilization, storage and shipment of DNA at room temperature
  • High yield DNA recovery and use without further purification
  • Compatible with most downstream applications, e.g., PCR, sequencing, and microarrays
  • Available in a variety of compact formats, e.g., tubes, multi-well plates & custom.
  • Cost-effective, eco-friendly solution to freezer crawl and lab space management

DNAstable® enables you to preserve, store and ship your purified DNA samples at room temperature. Sample recovery is achieved within minutes by simply adding water or buffer to the matrix. Sample is ready for downstream analysis without need for any additional purification step.

DNA stabilization can be achieved in two formats:


Pre-dried in tubes or plates (DNAstable)

DNAstable and DNAstable LD Workflow

 

Liquid-to-Dry storage format (DNAstable LD)

Product Performance

Successful PCR after long term storage in DNAstable:

Successful PCR after long-term storage of plasmid DNA in DNAstable
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Figure 1: Aliquots (1 ng) of pDNA, either stored in DNAstable (DS) or unprotected (NP) for 26 months at ambient temperature (RT) or at 60°C under accelerated aging conditions (equivalent to 30 years of room temperature storage) were analyzed by PCR amplification using pUC19 specific primers generating a 490 bp amplicon. Reference DNA samples (1 ng) were stored at -20°C (control).

Stability in Liquid-to-Dry (LD) Format:

Successful PCR after long-term storage of genomic DNA in DNAstable
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Figure 2: Two different aliquots (20ng) of gDNA stored in DNAstable (DS) at room temperature without humidiy control for 3.5 years were analyzed by PCR amplification using β-actin specific primers generating a 500bp amplicon. Reference DNA sample (100ng) was stored at -20°C.

Recovery of genomic DNA after 26 months of storage in DNAstable

Genomic DNA recovery after 26 months in DNAstable
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Figure 3: Genomic DNA samples were stored for 26 months in a number of conditions, recovered and compared. From left to right: Control -20°C; Stored in DNAstable (DS) at room temperature (RT); Unprotected (NP) at room temperature; Stored in DNAstable (DS) for the equivalent of 30 years at room temperature (exposed to accelerated aging conditions at 60°C); 5) Unprotected (NP) and stored for the equivalent of 30 years at room temperature (exposed to accelerated aging conditions at 60°C).

qPCR of genomic DNA stored for 26 months in DNAstable

qPCR with genomic DNA recovered after 26 months in DNAstable
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Figure 4: qPCR traces of experiments described in Figure 3 above with standard deviation displayed.

Product Specifications


Product formats
  • 1.7ml centrifuge tubes
  • 96 and 384 well-plates
  • 0.5ml 2D barcoded and alphanumeric 96 tube-plates
  • Custom
Application volume 5 to 150μl
Application amount 0.001 to 30μg
Drying time 10 min (SpeedVac) to overnight (Laminar hood)
Recovery volume 10 to 100μl
Recovery ∼99.9%
DNA long-term storage 30 years accelerated aging; ∼4 years ″real time″
DNA stabilization testing temperature Temperature > 60°C
Resources
 
DNAstable® / DNAstable® Overview
DNAstable® / DNAstable® LD Handbook
DNAstable® 1.5-ml Flip-Cap Tube, 96-well and 384-well Plate Protocol
DNAstable® 96-well tube plates (2D barcode & Alphanumeric) Protocol
DNAstable® LD Protocol
DNAstable® MSDS
DNAstable® LD MSDS
96-well plate design diagrams
Application Notes
Evaluation of Biomatrica DNAstable® room temperature DNA storage system - 12month and 45month data.
Optimizing storage and handling of DNA extracts
DNAstable® protects genomic DNA from degradation under high pressure and extreme temperatures above 120°C
DNAstable® protects plasmid DNA stressed at high temperature
DNAstable® protects plasmid DNA from UV-C irradiation
DNAstable® preserves plasmid DNA for long-term storage
Long-term storage of oligonucleotides in DNAstable®
DNAstable® with the Nanogen® Genotyping System
Performance of DNAstable® with the Illumina® Human 610 BeadChip™

Ordering

Catalog No. Product Description Size
93021-001 DNAstable® Tube Kit 25 tubes of DNAstable
93021-011 DNAstable® (3 x 25) Tube Kit 3 x 25 tubes of DNAstable
90021-001 DNAstable® 96-Well Plate 1 DNAstable 96-well plate
90022-001 DNAstable® 96-Well 10 Plate Kit 10 DNAstable 96-well plates
91021-001 DNAstable® 384-Well Plate 1 DNAstable 384-well plate
90031-006 DNAstable® 96-Tube Plate (Matrix) 96 DNAstable alphanumeric tubes in a 96-well holding rack
90041-006 DNAstable® 2D-Barcoded Tubes (Matrix) 96 DNAstable 2D barcoded tubes in a 96-well holding rack
53001-066 DNAstable® LD 2 ml
52001-026 DNAstable® LD 10 ml
52091-036 DNAstable® LD 50 ml
52091-037 DNAstable® LD 5 x 50 ml
93000-001 DNAstable® Trial Kit 3 tubes of DNAstable

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