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DNAstable® Blood

Stabilize and Preserve DNA in Whole Blood at Room Temperature
  • Preserve DNA in blood and buffy coat samples for at least 12 years at ambient temperatures.
  • Cost effective and convenient blood sample archiving
  • Compatible with all downstream applications

Cost Effective Solutions to Store Blood Samples

DNAstable® Blood is an innovative product for dry storage and preservation of DNA in blood samples at ambient temperatures. The DNAstable Blood matrix forms a thermostable barrier as samples dry, protecting the blood DNA from degradation. DNAstable Blood is available pre-dried in tubes. Once the DNAstable Blood reagent is mixed with blood, samples can be dried in a laminar flow hood at room temperature, in a ventilated 40°C incubator, or in a vacuum centrifuge. Samples can then be stored at ambient temperature for at least 12 years (based on accelerated aging studies).

DNAstable Blood - 12 years at room temperature

Product Performance

No degradation of blood DNA after 12 years of storage*

Agarose gel showing DNA stabilized in DNAstable Blood (DGB) maintains integrity vs. non-protected negative control and -20 degree C positive control
Figure 1. Human whole blood samples (200 µl) were stored for 31 months under various conditions: storage at –20°C (Control); storage in DNAstable Blood at 25°C or 45°C (equivalent to approx. 12 years of storage at room temperature)1; or storage at 45°C without protection (NP). After blood rehydration, genomic DNA was purified using the QIAamp DNA Blood Mini Kit. DNA eluates (10 µl) were analyzed on a 0.8% agarose gel. M: markers.

Higher DNA recovery from whole blood stored in DNAstable blood vs FTA paper

agarose gel showing higher DNA recovery vs. FTA paper
Figure 2. Duplicate Human whole blood samples (200 µl) were stored for 12 months under various conditions: storage at –20°C (Control); storage in DNAstable Blood at 45°C (equivalent to approx. 5 years of storage at room temperature);* or storage at 25°C on FTA paper (FTA). After blood rehydration, genomic DNA was purified using the QIAamp DNA Blood Mini Kit. DNA eluates (10 µl) were analyzed on a 0.8% agarose gel. M: markers. extraction. (M = 1kb ladder).

No degradation of buffy coat DNA after 11 years of storage*

agarose gel showing higher DNA recovery vs. FTA paper
Figure 3. Human buffy coat samples (200 µl) were stored for 27 months under various conditions: storage at –20°C (Control); storage in DNAstable Blood at 45°C (equivalent to approx. 11 years of storage at room temperature)1. After buffy coat rehydration, genomic DNA was purified using the QIAamp DNA Blood Mini Kit. DNA eluates (10 µl) were analyzed on a 0.8% agarose gel. M: markers.

Broad Range of Applications

Successful long-range PCR after 3 years of storage*

Agarose gel showing integrity of DNA stored in DNAstable after 3 years of storage
Figure 4. Whole blood samples (200 µl) from the same donor were stored for 9 months at –20°C or in DNAstable Blood Tubes at 25°C or 45°C (equivalent to approx. 3 years of storage at room temperature).* After blood rehydration, genomic DNA was purified using the QIAamp® DNA Blood Mini Kit. DNA eluates (2 µl) were then used in PCR of a 5 kb DNA fragment of the human genome using the QIAGEN® LongRange PCR Kit. The PCR products were visualized on a 0.8% agarose gel. M: 1 kb Plus Ladder.

Reliable polymorphism detection using melting curve analysis after 9 months of storage

Image of DNA eluates analyzed using the artus MTHFR LC PCR Kit
Figure 5. Human whole blood samples (200 µl) were stored for 9 months at –20°C or in DNAstable Blood Tubes at room temperature. After blood rehydration, genomic DNA was purified using the QIAamp DNA Blood Mini Kit. DNA eluates (2 µl) were then analyzed using the artus® MTHFR LC PCR Kit. ‡ Both samples showed no significant difference in melting curve analysis and indicated a heterozygous variant.

*Accelerated aging studies: A temperature increase of 10°C corresponds to a twofold increase in shelf life. For example, DNA stored at 40°C for 2 years corresponds to an estimated storage time of 8 years at 20°C.

Product Specifications

DNAstable Blood

Product formats Tubes
Application volume 1 - 200µL of blood
DNA Recovery ~99.9%
Blood DNA long-term storage At least 12 years at ambient temperatures

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Ordering

Catalog No. Product Description Size Price
93027-027 DNAstable® Blood Tubes 50 tubes of DNAstable Blood $295

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