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Overview

Long term storage of Whole Blood at Room Temperature.

Biomatrica® has developed a new stabilization product that enables you to preserve DNA in blood and buffy coat samples for at least 12 years at room temperature. You recover all of your samples in minutes.

Technology Overview:

Features & Benefits Protection and stabilization of DNA in blood at room temperature Convenient archiving of blood samples Cost effective sample storage Environmentally friendly: No energy required Compatible with all downstream applications

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Stabilization of DNA at Room Temperature - Convenient archiving.

DNAstable Blood provides innovative technology for dry storage of bood samples at room temperature. The DNAstable matrix forms a thermostable barrier round the sample as it dries. Drying occurs in a laminar flow hood at room temperature or in a ventilated 40°C incubator. Samples can then be stored at ambient temperature for at least 12 years (based on accelerated aging theory).


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Figure 1. No degradation of blood DNA after 12 years of storage*. Human whole blood samples (200 μl) were stored for 31 months under various conditions: storage at –20°C (Control); storage in DNAstable Blood at 25°C or 45°C (equivalent to approx. 12 years of storage at room temperature)1; or storage at 45°C without protection (NP). After blood rehydration, genomic DNA was purified using the QIAamp DNA Blood Mini Kit. DNA eluates (10 μl) were analyzed on a 0.8% agarose gel. M: markers.
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Figure 2. Higher DNA recovery from whole blood stored in DNAstable blood vs FTA paper. Duplicate Human whole blood samples (200 μl) were stored for 12 months under various conditions: storage at –20°C (Control); storage in DNAstable Blood at 45°C (equivalent to approx. 5 years of storage at room temperature);* or storage at 25°C on FTA paper (FTA). After blood rehydration, genomic DNA was purified using the QIAamp DNA Blood Mini Kit. DNA eluates (10 μl) were analyzed on a 0.8% agarose gel. M: markers. extraction. (M = 1kb ladder)..

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Figure 3. No degradation of buffy coat DNA after 11 years of storage*. Human buffy coat samples (200 μl) were stored for 27 months under various conditions: storage at –20°C (Control); storage in DNAstable Blood at 45°C (equivalent to approx. 11 years of storage at room temperature)1. After buffy coat rehydration, genomic DNA was purified using the QIAamp DNA Blood Mini Kit. DNA eluates (10 μl) were analyzed on a 0.8% agarose gel. M: markers.

Broad range of applications

DNA protected using DNAstable Blood is suitable for use in any downstream applications, e.g. long-range PCR, real time PCR, sequencing, etc.

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Figure 3A . Successful long-range PCR after 3 years of storage*. Whole blood samples (200 μl) from the same donor were stored for 9 months at –20°C or in DNAstable Blood Tubes at 25°C or 45°C (equivalent to approx. 3 years of storage at room temperature).* After blood rehydration, genomic DNA was purified using the QIAamp® DNA Blood Mini Kit. DNA eluates (2 μl) were then used in PCR of a 5 kb DNA fragment of the human genome using the QIAGEN® LongRange PCR Kit. The PCR products were visualized on a 0.8% agarose gel. M: 1 kb Plus Ladder.

Representative DNA yield

DNA protected using DNAgard is suitable for use in many downstream applications, e.g. long-range PCR, real time PCR, etc.

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Figure 4. Reliable polymorphism detection using melting curve analysis after 9 months of storage.Human whole blood samples (200 μl) were stored for 9 months at –20°C or in DNAstable Blood Tubes at room temperature. After blood rehydration, genomic DNA was purified using the QIAamp DNA Blood Mini Kit. DNA eluates (2 μl) were then analyzed using the artus® MTHFR LC PCR Kit. ‡ Both samples showed no significant difference in melting curve analysis and indicated a heterozygous variant.

 

*Accelerated aging studies: A temperature increase of 10°C corresponds to a twofold increase in shelf life. For example, DNA stored at 40°C for 2 years corresponds to an estimated storage time of 8 years at 20°C.