Preserve RNA Instantly
The RNAgard® Blood System is designed for the collection, preservation and purification of RNA from whole blood samples. The RNAgard® Blood preservative reagent in the VACUETTE® tubes rapidly permeates and lyses cellular structures and membranes to stabilize and protect RNA from degradation. Preservation is effective for up to 14 days at room temperature and 1 month at 4°C.
RNAgard® and BioMaxi® are registered trademarks of Biomatrica. VACUETTE® is a registered trademark of Greiner Bio-One. RNAgard Blood System is for Research Use Only. Not for use in diagnostic procedures.
Figure 1: Multiple donor comparison of RNAgard Blood Tubes (RGB) versus Competitor P (P) after 14 days of ambient temperature storage. 200ng of RNA is visualized on a 1.2% agarose gel. BioMaxi Blood RNA Purification kit was used for isolating RNA from RNAgard Blood Tubes.
Figure 2: RNA yield analyzed by UV spectroscopy. RNA amounts are for whole tube (2.5 ml blood) isolating using BioMaxi Blood RNA Purification kit after 14 days of storage at ambient temperature.
Figure-3: Gene expression analysis of blood RNA preservation after 3 and 14 days in RNAgard Blood (RGB). Human blood from 2 healthy donors was collected in RNAgard tubes or competitor’s RNA preservation tubes. RNA was isolated on collection day and after 3 or 14 days of room temperature storage. Top: Representative analysis, for Donor 2, of gene expression profile of more than 47000 transcripts, using the Ilumina Human HT12 Bead Array, for RNA extracted from blood collected in RNAgard Blood or competitor’s Tubes after 3 days (A,C) or 14 days (B,D) of room temperature storage, relative to RNA extracted from freshly collected blood, using RNA isolation methods recommended for each product. Bottom: Table summarizing the microarray results. Displayed are the number of genes with > 2-fold change in expression levels relative to expression levels on the day of blood sample collection.
Figure-4: RNA purity obtained using the RNAgard Blood System is highly reproducible. Blood was collected from 8 healthy donors. After 3, 7 or 15 days of sample storage, RNA was purified from duplicate samples. RNA purity of each individual sample was determined by UV spectrophotometry (A260/A280). On Day 3, duplicate samples for each donor were purified by 3 different operators showing reproducibility of our system.
Figure-5: RNAgard Blood Tube maintains unaltered gene expression for 14 days at 18-25°C. Blood from two healthy donors was collected in RNAgard tubes and purified with BioMaxi RNA after the indicated storage time. Gene expression of C-Fos (A) and IL1-β (B), normalized to 18S rRNA, was determined by qPCR at the indicated storage time, relative to day 0 samples.
Figure-6: RNA yield of whole blood from 5 different donors was tested using the RNAgard Blood System following storage for 7 weeks at 4°C versus control (Day = 0). Data shows that the quality and yield of RNA following 7 weeks of storage in RNAgard Blood Tubes at 4°C were equivalent to that of RNA purified from whole blood on the day of blood draw.
|Product Overview Sheet|
|MSDS - RNAgard Blood System|
|MSDS - VACUETTE RNAgard Blood Tubes|
|MSDS - BioMaxi Blood RNA Purification Kit|
|Graphical Instructions for Use (IFU)|
|RNAgard Blood System Poster|
|Automated purification of RNA from RNAgard Blood Tubes using QIAcube® sample prep platform|
|Automation of RNA isolation from blood stabilized in RNAgard® Blood Tubes with the Roche MagNA Pure Compact System|
|Automated purification of RNA from RNAgard Blood Tubes using the Agencourt RNAdvance® Blood System|
|RNA isolation from RNAgard Blood Tubes using a modified version of the RiboPure™ Blood Kit|
|RNAgard Application Note|
|Catalog No.||Product Description||Size|
|45201-000||RNAgard® Blood System||50 reactions|
|62201-131||VACUETTE® RNAgard® Blood Tubes||50-pack|
|62201-141||VACUETTE® RNAgard® Blood Tubes||1200-pack|
|64201-601||BioMaxi® Blood RNA Purification Kit||50 preps|
|RP1-001-FG||BioMaxi® Precipitation Buffer||50 preps|
|RS1-001-FG||BioMaxi® Resuspension Buffer||50 preps|