Enabling total mRNA Recovery in Sequencing for Immuno-Oncology

Customer Problem

  • Adaptive Biotechnologies needs concentrated mRNA purified from T-cells for cDNA synthesis and sequencing of T-cell receptor α β gene pairs using their pairSEQ™ technology.
  • Filter concentration causes mRNA loss, compromising accuracy.
 

Biomatrica Solution

  • RNAstable® effectively eliminates mRNA loss using simple drying and rehydration of mRNA to achieve desired volume.
  • Use of RNAstable ensures that all cognate T-cell receptor mRNA species are converted to cDNA and sequenced.
 

Customer Impact

  • RNAstable enables Adaptive Biotechnologies to integrate mRNA purification with cDNA synthesis in one workflow, facilitating sequencing of T-cell receptor genes, critical to pairSEQ technology.
  • RNAstable adds robustness to a workflow with applications in immuno-oncology research, clinical diagnostics and therapeutics.
Pair-SEQ workflow with-RNAstable
 

References

  • Adaptive will use pairSEQ in “drug development efforts…starting with cancer,” Chad Robins, CEO, Adaptive Biotechnologies.
  • Howie, B,, Sherwood, A.M., Berkebile, A.D., Berka, J., Emerson, R.O., Williamson, D.W., Kirsch, I., Vignali, M., Rieder, M.J., Carlson, C.S., Robins, H.S. High-throughput pairing of T cell receptor α and β sequences. Sci Transl Med. 2015 Aug 19;7(301):301ra131. doi: 10.1126/scitranslmed.aac5624.

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