CTC Preservation in Blood
- Normalized EpCAM positive cells on Day 2 divided by EpCAM positive cells on Day 0 of no formulation control.
- Percent of 7AAD negative, EpCAM positive cells on Day2.
- Mean fluorescence intensity of Day 2 EpCAM positive cells divided by Day 0 EpCAM positive cells with no formulation control.
- ΔΔCt from Day 0 of ΔCt values normalized to actin.
CTC Stabilization Preservation of CTC’s spiked into blood
samples. CTC spiked blood samples were stored
for 4 days at ambient temperature with or without preserving formulations. CTC’s in unprotected blood (NP) deteriorated after a day, where CTC’s in preserved blood were intact after 4 days.